» Educational Qualifications

Bachelor`s Degree » Graduated in 1993
Master`s Degree » Graduated in 1996
Doctoral Degree » Graduated in 2003

Trakya University Institute of Science, Department of Chemistry

Doctoral Degree Thesis;

Interest in lipases has markedly increased to their potential industrial applications. Because of the interest and largest producer of Turkey, In this study, it was aimed that Lipase from hazelnut seed identified as yomra species isolated, purified and characterized. Lipase from hazelnut seed was purified 1255 fold to homogeneous state by ammonium sulfate precipitation, dialysis and Sephadex G-100 gel filtration chromatography after by defatting from hazelnut proteins. The purified enzyme showed single band when it was subjected to SDSPAGE. The molecular weight determined by SDS-PAGE was 20 kDa. Purified lipase from hazelnut seed exhibited the maximum activity at pH 9.0 and 50˚C, stable under alkaline conditions (pH 7.0-10.0) and at temperatures between 20-55˚C. Lipase from hazelnut seed was determined more specific versus triolein, tributyrin and olive oil among the natural oils as substrate. To determine the storage stability of lipase from hazelnut seed, the activity assays carried out for a period of one year. it was observed that about 83% of its activity was retained of 9 months at -20˚C. Then the lipase activity retains constant. In the presence of 1.0mM CaCl2, the lipase activity was considerably increased by 77%. Km and Vmax values of purified lipase from hazelnut seed versus triolein as substrate calculated 4.545mM and 80 U/dk.mg.Enzyme, respectively.

» Publications

SCI-SSCI or SCI Expanded Articles

.I. Kilic, Spectrometric Studies on Antioxidant Activity of Pinus Nigra Resin In Vitro and Its Total Phenolic and Flavonoid Content, 10.2174/1573412911666151022193432, CURRENT PHARMACEUTICAL ANALYSIS, vol. 12, no. 2, pp. 146-151, 2016.

.I. Kilic, A. Moralar, The Effect of Problem-Based Learning Approach on Academic Success and Motivation in Science Education, 10.14527/pegegog.2015.034, PEGEM EGITIM VE OGRETIM DERGISI, vol. 5, no. 5, pp. 625-636, 2015.

.I. Kilic, Y. Yesiloglu, Y. Bayrak, Investigation on the Antioxidant Activity of Roots and Stem of Colchicum turcicum L., ASIAN JOURNAL OF CHEMISTRY, vol. 26, no. 1, pp. 5-9, 2014.

.I. KILIÇ, Y. YESILOGLU, Y. BAYRAK, Spectroscopic studies on the antioxidant activity of ellagic acid, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 130, pp. 447-452, -AMERIKA BIRLESIK DEVLETLERI, 9, 2014.

.I. KILIÇ, Y. YESILOGLU, Y. BAYRAK, Investigation on the Antioxidant Activity of Roots and Stem of Colchicum turcicum L, Asian Journal of Chemistry, vol. 26, no. 1, pp. 5-9, 1, 2014.

.Y. Yesiloglu, L. Sit, I. Kilic, in vitro Antioxidant Activity and Total Phenolic Content of Various Extracts of Satureja hortensis L. Collected from Turkey, ASIAN JOURNAL OF CHEMISTRY, vol. 25, no. 15, pp. 8311-8316, 2013.

.Y. Yesiloglu, H. Aydin, I. Kilic, In Vitro Antioxidant Activity of Various Extracts of Ginger (Zingiber officinale L.) Seed, ASIAN JOURNAL OF CHEMISTRY, vol. 25, no. 7, pp. 3573-3578, 2013.

.I. Kilic, Y. Yesiloglu, Y. Bayrak, S. Gulen, T. Bakkal, Antioxidant Activity of Rumex conglomeratus P. Collected from Turkey, ASIAN JOURNAL OF CHEMISTRY, vol. 25, no. 17, pp. 9683-9687, 2013.

.Y. Bayrak, H. Topallar, B. Karagoz, I. Kilic, Kinetics and Thermodynamics of Cr(VI), Cu(II), and Ni(II) Adsorption on Activated Carbon Prepared from Rice Hulls, 10.1080/01932691.2012.743347, JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, vol. 34, no. 9, pp. 1248-1256, 2013.

.I. Kilic, Y. Bayrak, H. Topallar, B. Karagoz, Preparation of Rice Hull-Based Activated Carbon with Ammonium Nitrate, ASIAN JOURNAL OF CHEMISTRY, vol. 25, no. 9, pp. 4911-4916, 2013.

.I. Kilic, Y. Yesiloglu, Spectroscopic studies on the antioxidant activity of p-coumaric acid, 10.1016/j.saa.2013.06.110, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, vol. 115, pp. 719-724, 2013.

.Y. Yesiloglu, I. Kilic, Lipase-catalyzed esterification of glycerol and oleic acid, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, vol. 81, no. 3, pp. 281-284, 2004.

Polyvinyl Alcohol-Trypsin as Catalyst for Amino Acid Ester Synthesis in Organic Media” Preparative Biochemistry and Biotechnology, 34(4), 365-375, (2004).

Lipase-Catalyzed Esterification of Glycerol and Oleic Acid” JAOCS, 81(3), 281-284, (2004).

International Conference Proceedings

.KILIÇ ISMAIL,Gürbüz Özkan, The Effect of Science Instruction Supported by Educational Games on the Motivation of Secondary School Students within the Scope of Light Spread Unit, International Conference on Science, Mathematics, Entrepreneurship andTechnology Education, pp. 150-150, IZMIR-TÜRKIYE.

.I. KILIÇ, Ö. GÜRBÜZ, “Isigin Yayilmasi” Ünitesi Kapsaminda Egitsel Oyunlar Ile Desteklenmis Fen Ögretiminin Ortaokul Ögrencilerinin Motivasyonu Üzerine Etkisi, Uluslararasi Fen, Matematik, Girisimcilik ve Teknoloji Egitimi Kongresi, pp. 458-463, IZMIR-TÜRKIYE.

.I. KILIÇ, S. ERGÜN, The Effects of Computer Assisted Collaborative Learning Method in Science and Technology Classes on Students’ Attitudes, 13th International Balkan Education and Science Congress, pp. 151-.

.I. KILIÇ, Y. YESILOGLU, Free radical scavenging activity of p coumaric acid, 10. Chemistry Conference, Plovdiv-BULGARISTAN.

International Books and Book Chapters

.KILIÇ ISMAIL, Atilkan Furkan, Research & Reviews in Educational Sciences, Gece Kitapligi, Ankara-TÜRKIYE.

.KILIÇ ISMAIL, INSAC Social and Education Sciences, Duvar Kitabevi, ANKARA-TÜRKIYE.

.KILIÇ ISMAIL, Gürbüz Özkan, INSAC Advances in Social and Education Sciences, Duvar Kitabevi, Izmir-TÜRKIYE.

National Articles

.KILIÇ ISMAIL, ÖZMEN ULU Melike, Examination of Teacher, Student and Parents’ Views in The Implementation of Project Based Learning Approach in Life Science Lesson, Trakya Üniversitesi Sosyal Bilimler Dergisi, vol. 23, no. 1, pp. 449-470, 2021.

.I. KILIÇ, A. MORALAR, Fen egitiminde probleme dayali ögrenme yaklasiminin akademik basari ve motivasyona etkisi, Pegem Egitim ve Ögretim Dergisi, vol. 5, no. 5, pp. 625-636, ANKARA-TÜRKIYE, 11, 2015.

National Conference Proceedings

.I. KILIÇ, Y. YESILOGLU, p Kumarik Asitin Antioksidan Özelliklerinin Belirlenmesi, 27. Ulusal Kimya Kongresi, pp. 166-, ÇANAKKALE-TÜRKIYE.

.I. KILIÇ, A. SAGIROGLU, Düsük Molekül Agirlikli Polipeptidlerin Sodyum Dodesil Sülfat Poliakrilamid Jel Elektroforezi ile Ayrilmasi, XI. Ulusal Kimya Kongresi,, pp. 237-, VAN-TÜRKIYE.

» Biography

» Educational Qualifications

Bachelor`s Degree » Graduated in 1993

Master`s Degree » Graduated in 1996

Doctoral Degree » Graduated in 2003

Doctoral Degree Thesis;